Recombinant protein expression and purification experiments based on SARS-CoV-2 Mpro

نویسندگان

چکیده

Mpro is one of the most important drug targets novel Coronavirus. In this study, a series potential compounds were obtained through virtual screening targeting Mpro. Using key protease (Mpro) SARS-COV-2 as lead target for has become hot topic in current virus research. plays role replication and transcription. Therefore, experimental strain Escherichia coli containing commonly used to inhibit drugs from gene was located on peT-28A vector, size recombinant protein about 36 kDa. The e. Coli expression BL21(DE3) inoculated cultured.

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ژورنال

عنوان ژورنال: E3S web of conferences

سال: 2021

ISSN: ['2555-0403', '2267-1242']

DOI: https://doi.org/10.1051/e3sconf/202126702023